In addition, the immune‐killing can be initiated by releasing the cytotoxic granules including pore‐forming perforin proteins and serine proteases‐granzymes (especially granzyme B, GrB) to lyse tumor cells.[10] The perforation is a decisive and membrane‐disruptive step to form pores on tumor cells[11] and assist GrB for inducing cell death.[10, 12]. This evidence concerns the gene PRF1 and neoplasm.