ISLR and familial dilated cardiomyopathy: We identified the top eight hub genes (LTBP2, ASPN, ISLR, MFAP4, FNDC1, PODN, HTRA1, and FAP) using three different algorithms in CytoHubba (Supplementary Figure S3), and RT-qPCR was used to further analyze these hub genes in a DOX-induced DCM animal model (Figure 3; Supplementary Figure S4).