From the prioritized and other genes, six DEGs (BLNK, CCR4, ILR6, MICB, RIPK2, and TNFSF10) and two housekeeping genes (ACTB and GAPDH) were chosen to validate study results on a larger cohort of 162 tissue samples, including 91 CRC tissues and 71 adjacent tissues. The gene discussed is ACTB; the disease is colorectal carcinoma.