Metacluster (mc)2 and mc3 were uniquely expressed in peripheral blood (mc2 p=0.03 for PBMC vs ascites and p=0.04 for PBMC vs tumor, mc3 p=0.002 for PBMC vs ascites and p=0.003 for PBMC vs tumor) and included CD4+ T lymphocytes with naïve (TN, CD45RA+CD62L+CD95-, mc3) and central memory (TCM, CD45RA-CD62L+CD95+, mc2) phenotype, while mc8 was expressed in both PBMC and ascitic compartments (p=0.03 for PBMC vs tumor) and was composed by late-stage (TEMRA, CD45RA+CD62L-) CD4+ T cells. Here, CD4 is linked to neoplasm.