A Lm-based hepatocellular carcinoma (HCC) vaccine, ΔdalΔdat Lm-multiple peptides fusing genes (MPFG), with the ability to secrete HCC-related TAAs fragments, activates the TAMs through the NF-κB pathway and shifts the cytokine profiles within TME towards an antitumor response. The gene discussed is NFKB1; the disease is hepatocellular carcinoma.