We then examined mRNA expression levels for the four selected GRAs (GRA1, GRA2, GRA3, and GRA7), one ROP (ROP35), and one control molecule (SAG2C) of T. gondii molecules between the cysts that persisted in the presence and the absence of the perforin-mediated anti-cyst activity in CD8+ immune T cells. The gene discussed is PRF1; the disease is cyst.