In addition, Atg7, TSC2 and Beclin-1 have shown similar results to those of pharmacological inhibition, and allicin-induced colocalization of LC3-II-FITC punctate and mitochondria could be suppressed by 3-MA (autophagy inhibitor) pretreatment, which confirmed the induction of autophagy by allicin in HepG2 human liver cancer cells (7). The gene discussed is TSC2; the disease is liver cancer.