Contrary to our results, Araujo et al. [25] observed higher levels of RASSF1A methylation in HCC tissues of the younger group (<40 years) which could be explained by observing that all HCC patients under 40 years of age in their study were chronically infected with HBV, possibly infected a long time ago through vertical transmission. Here, RASSF1 is linked to hepatocellular carcinoma.