We used CD#59 and CD#68 iPSCs derived from fibroblasts of two CD patients with ASPA gene mutation(s) and a rescued iPSC line (CD#68 ASPA) with the wildtype (WT) ASPA gene knocked into the AAVS1 safe harbor site in CD#68 iPSCs generated in our previous study.30 The gene discussed is PPP1R12C; the disease is Cowden disease.