TNF and adenocarcinoma: Immunofluorescent staining of PCa tissue microarrays (TMA) was performed to determine whether there is a correlation between M. hyorhinis infection and TNF-α expression using 72 tissue samples from 20 patients on TMAs (16 cases of adenocarcinoma, 8 cases of adjacent tissue, 4 cases of normal tissue, duplicate or triplicate cores per case) (Fig. 2A).