In addition, PC3-M and C4–2B-M cells expressed significantly high levels of TNF mRNA and TNF-α protein compared to their parental cells (PC3–P and C4–2B–P) and the triple-negative breast cancer cell line, MDA-MB-231 that is known to make TNF-α (Fig. S1). This evidence concerns the gene TNF and triple-negative breast carcinoma.