Here, by crossing Srsf2Floxed/Floxed (Srsf2F/F) mice with Stra8-Cre mice to generate mutant mice with specific deletion of the Srsf2 gene in male germ cells, we found that the SRSF2 knockout caused complete infertility and germ cells were drastically lost during spermatogenesis. This evidence concerns the gene SRSF2 and Infertility.