Conversely, compared with P-MSC group, CSF2 knockdown reduced the effects of P-MSCs in promoting gastric cancer cell viability (Fig. 2E), cell colony formation (Fig. 2F), migration (254.83 ± 9.82 vs. 165.00 ± 7.78) (Fig. 2G) and reducing IC50 of 5-FU (126.74 ± 4.56 vs. 79.26 ± 3.01) (Fig. 2H) and apoptotic cell rate (4.04 ± 0.50% vs. 8.85 ± 0.69 %) (Fig. 2I). This evidence concerns the gene CSF2 and gastric cancer.