Ad-CMV-Cre, but not Ad-CMV-GFP, infection led to the inversion of the conditional by inversion (COIN) module and expression of the Notch2ΔPEST or Notch2ΔINV mRNA with the consequent induction of Hes1, Hey1, and Hey2 demonstrating activation of Notch signaling (Fig. 4). This evidence concerns the gene HEY2 and infection.