MKNK1 and colorectal carcinoma: After 6 Gy irradiation, a colony formation assay was carried out to verify the results, which indicated that the cell clone formation rate in the second group was substantially elevated than the first group, while its rate in the third group was notably lower than the second group (Figure 6A,B), suggesting that tRF‐16‐7X9PN5D stimulates radioresistance in CRC cells by directly targeting MKNK1.