Western blotting showed that the protein levels of the RhoA-ROCK-β-catenin signal axis and Phenotypic associated protein (Bcl-2, α-SMA, Collagen I, N-Cad and Vimentin) increased to various degrees for T-BPH rat prostates, while BAX and E-Cad decreased. The gene discussed is VIM; the disease is benign prostatic hyperplasia.