Furthermore, the in vivo inactivation of STK25 and MST3 has been shown to effectively hinder the initiation and aggravation of NAFLD in obese mice, and to dampen the development of diet-induced systemic hyperinsulinemia (Cansby et al. 2019b; Nunez-Duran et al. 2018; Amrutkar et al. 2015a; Caputo et al. 2021b). This evidence concerns the gene STK25 and metabolic dysfunction-associated steatotic liver disease.