By analyzing Dicer’s expression changes in different grades of glioma in the CGGA database, it was found that the changing trend was consistent with that of tRF-5s such as tRF-19-R118LOJX, suggesting that the down-regulated Dicer in the GBM group might be one of the reasons for reducing biogenesis of tRF-5s. This evidence concerns the gene DICER1 and glioblastoma.