To further analyze the effects of HMGB1 inhibitor treatment in APOE-tauopathy mice, we performed single-nucleus RNA sequencing (snRNA-seq) on hippocampi isolated from 9.5-month-old PS19-E4 and PS19-E3 mice that underwent a 12-week treatment with the HMGB1 inhibitors or saline (Figure S7A). This evidence concerns the gene APOE and tauopathy.