TP53 and cancer: To conclude, the CAM can be a promising but challenging model for mass manufacturing of recombinant viruses such as HSV-1-P53 which are not able to replicate in common cell lines, but whether restoration of wild-type P53 activity by HSV-1 oncolytic would be a potential approach for triggering the p53-mediated pro-apoptotic and enhancing the oncolytic potency in almost all human cancers, deserves further studies.