CALCA and inflammatory response: Immunoblot analyses of LBP, which reflects LPS in the liver tissues, F4/80, which indicates the infiltration of macrophages into livers (i.e. KC infiltration), and HEL, which is an oxidative stress marker of early lipid peroxidation, were performed to investigate the molecular mechanism related to how Nrf2 in macrophages protects against hepatic inflammation and fibrosis.