Furthermore, western blotting assay was performed, and result suggested that the protein level phosphorylation-ERK1/2 (p-ERK1/2) was suppressed in osteosarcoma cells when HSPB6 was overexpressed, whereas the protein level of ERK1/2 did not vary significantly (Fig. 4C, D). Here, HSPB6 is linked to osteosarcoma.