Some studies have identified that the application of MIF inhibitors hindered the conduction of the NF-kB signal,[29,30] and overexpression of MIF facilitated the progression of osteosarcoma through activating the Ras signal pathway.[31] Moreover, our analysis revealed that CD8T cell marker genes were enriched in NF-kB and the Ras signal pathways. The gene discussed is NFKB1; the disease is osteosarcoma.