During inflammation, cytokines like TNF-α and IFN-γ trigger the induction of iNOS, which in turn boosts the production of nitric oxide.[50] Furthermore, a reduction in antioxidant defense enzymes, particularly superoxide dismutase and glutathione peroxidase, was identified in patients with BD based on a cross-sectional study.[51] Our study revealed that the fluid shear stress and atherosclerosis pathways were the most significantly enriched pathways according to KEGG analysis. This evidence concerns the gene TNF and atherosclerosis.