The possible explanations for discrepancies in test results obtained by CareStart RDT and microscopic examination due to microscopic examination of Giemsa-stained thick or thin blood smears remains the superior diagnostic method for identifying species and stages of the Plasmodium parasites.[12,19] The accuracy of malaria diagnostics depends on several factors, including the level of malaria endemicity, parasite density, mutation or deletion of the gene encoding the HRP2, format and type of the RDT product, and storage conditions.[13,21]. The gene discussed is HDGFL2; the disease is malaria.