In the diagnostic set, we analysed cfDNAme patterns in 27 ovarian cancer cases, four healthy volunteers, and 37 women with benign pelvic pathologies via targeted bisulfite sequencing of the three regions EpiFem-Care (EFC) 144, EFC 204 and EFC 228 located in the genes ZNF154, C2CD4D and WNT6, respectively (Figure 2 and Table S2). Here, ZNF154 is linked to ovarian carcinoma.