In fact, Gurbatri and collaborators showed in the 4T1 mouse model of breast cancer that local delivery of anti-PD-L1 with the use of synchronized lysis circuit (SLC) plasmid system led to no significant toxicities, while the systemic administration of anti-PD-L1 led to severe toxicities and death (62). The gene discussed is CD274; the disease is breast carcinoma.