When linc01224-shRNAs (sh-linc01224-1, sh-linc01224-2 and sh-linc01224-3) were targeted, the expression of linc01224 was downregulated in FaDu cells compared with negative control FaDu cells (shRNA-NC) and blank control (BC) FaDu cells by qRT-PCR. This evidence concerns the gene LINC01224 and breast cancer.