Variability in EZH2 staining in tumours was observed therefore EZH2 transcript levels were analysed using R2 Genomics Analysis and Visualization Platform using two gene expression datasets of RMS patients (ITCC n = 101, Barr n = 58 [2, 29]), and a dataset of childhood cancer cell lines (Versteeg n = 22), and compared to normal skeletal muscle samples (Asmann n = 40) [30]. The gene discussed is EZH2; the disease is neoplasm.