To demonstrate the changes in other CRC cell lines, expression of GLS1, PC and PDK1 was also investigated by immunoblotting in LS174T and HCA7 cells after long-term aspirin exposure (Supplementary Fig. 3a), showing upregulation of GLS1GAC in LS174T, though this is not statistically significant, and significant downregulation of PC and PDK1 in HCA7 cells. This evidence concerns the gene GLS and colorectal carcinoma.