PDK1 and colorectal carcinoma: To demonstrate the changes in other CRC cell lines, expression of GLS1, PC and PDK1 was also investigated by immunoblotting in LS174T and HCA7 cells after long-term aspirin exposure (Supplementary Fig. 3a), showing upregulation of GLS1GAC in LS174T, though this is not statistically significant, and significant downregulation of PC and PDK1 in HCA7 cells.