To further determine the role of microvascular GLUT1 in postoperative cognitive impairment, we conditionally overexpressed endothelial GLUT1 by ICV injection of AAV9-ICAM2-GLUT1 14 days before surgery, and then examined the GLUT1 expression and evaluated the cognitive performance on POD 14 (Fig. 4A). Here, ICAM2 is linked to Cognitive impairment.