Other performed tests included investigation for antibodies against Anaplasma phagocytophilum and/or platys (not differentiated by the test), Borrelia burgdorferi, Ehrlichia canis, Ehrlichia ewingii, and Dirofilaria immitis, which were performed in 5/63 dogs (SNAP 4Dx plus, Idexx, Netherlands), investigation of serum antibodies against Leptospira (1/63 dogs), PCR for tick borne encephalitis virus and/or the agent causing Lyme disease (2/63), and serum antibody measurements for Toxoplasma gondii (IgG and IgM) and/or Neospora caninum (2/63). Here, CD40LG is linked to Lyme disease.