Therefore, the roles of SH2D1A and the involved pathways were explored via CCK-8 assay, Transwell assay, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and western blotting, and the relationship between the immune microenvironment and SH2D1A expression was explored to evaluate the value of SH2D1A as a biomarker for HCC. This evidence concerns the gene SH2D1A and hepatocellular carcinoma.