To further reveal the potential pathway that CANT1 might regulate the carcinogenesis and development of HCC, GSEA analysis was carried out on CANT1-related DEGs and demonstrated the positive correlation of CANT1 with kinesin, resolution of sister chromatid cohesion, DNA strand elongation, and activation of the pre-replication complex based on the Reactome pathway(Fig. 8A). The gene discussed is CANT1; the disease is hepatocellular carcinoma.