Considering that RNF13 mRNA did not increase in NASH, and previous study indicates that RNF13 protein undergoes extensive post-translational proteolysis in both lysosome and proteasome, which makes it rather unstable14, we first compared the stability of RNF13 in the setting of BSA or PAOA treatment by cycloheximide (CHX) chase assays. The gene discussed is RNF13; the disease is metabolic dysfunction-associated steatohepatitis.