As shown in Fig. 8b and Supplementary Fig. 8b, ISO not only stimulated the expression of the hypertrophy biomarkers ANP, BNP, and β-MHC but also increased the level of lnc4012 in a dose-dependent manner, indicating that upregulation of lnc4012 is indeed closely correlated with the increased hypertrophic gene transcription and the development of myocardial hypertrophy. This evidence concerns the gene NPPB and cardiac hypertrophy.