Furthermore, in line with the enrichment of TNF-α and NF-kB signaling pathways in LSCs identified by PROGENy analysis of differentially expressed genes, higher H3K27ac and H3K4me3 signals were present in the loci of TNF-α and NF-kB target genes in LSCs, as compared to in KCs, while these loci in KCs were repressed by H3K27me3 (S4C Fig). The gene discussed is TNF; the disease is dry eye syndrome.