FMR1 and Treacher-Collins syndrome: NE-100 potentiates seizures at a 25 mg/kg doseand induces seizures at 50 mg/kg and above.75 To determine suitable doses of NE-100 for evaluation in the AGSassay—doses of NE-100 that straddle the threshold dose forNE-100 causing seizures on its own—we tested NE-100 at sixdifferent doses, 5, 10, 15, 20, 30, and 50 mg/kg, in juvenile WT and Fmr1 KO mice, and observed them for 30 min following administration.Like a previous report,75 we observed that50 mg/kg NE-100 induced generalized TCS (Table 2), whereas the mice exhibited normal behaviorwhen administered the lower doses.