Pseudo-time analysis of the EVT1 and EVT2 subtypes suggested that CSH1 and HSPG2 may regulate EVT differentiation, and both are downregulated in preeclamptic EVT, in line with previous placenta transcriptomics of women with both early-onset and late-onset preeclampsia (30, 32). Here, HSPG2 is linked to preeclampsia.