To further explore the mechanism of the protective effect of CST3 on hyperbilirubinemia, we studied the autophagy pathway and found that CST3 could enhance autophagy flux in HT22 cells (Figure 3a–e) and the protective effect could be inhibited by autophagy inhibitor bafilomycin A1 (Figure 3f). Here, CST3 is linked to Hyperbilirubinemia.