Our results showed that the average Ct values of the candidate genes were between 8 and 27 in the human colonic epithelial cell (NCM460) and cancer cell lines (HT29, HCT116, SW480, SW620, DLD-1, LOVO and RKO), and the expression of 18S was the highest while GUSB was the lowest in all cells except for LOVO cell line (Fig. 2A–H). Here, GUSB is linked to cancer.