Cultures containing a SMV1-targeting plasmid pTSMV1_36 and cultures containing the plasmid pTSMV1_36 cloned with gp40 overexpression cassette (pTSMV1_36OE40) were infected by SMV1 at a multiplicity of infection (MOI) of ∼2, using ΔC1C2 strain that lacks any CRISPR–Cas immunity as the control strain. Here, PDPN is linked to infection.