Using increasing doses of the DNA damaging agent etoposide (Figure 3E) or 5-fluorouracil (Figure 3F), we generated survival curves for control and mutant TDP-43 ALS neurons and found that compared to control neurons, mutant TDP-43 ALS neurons displayed a reduction in survival following treatment with either DNA damaging agent. Here, TARDBP is linked to amyotrophic lateral sclerosis.