CSPG4 and melanoma: Next, we co-cultured the T-cell clones with either 293T cells transfected with HLA-C*07:01 or HLA-C*07:02 cDNA and loaded with the CSPG4:554-562 (9-mer) or CSPG4:553-562 (10-mer) peptide (Figure 2C) or cotransfected with CSPG4:1-562 cDNA (Figure 2D) or co-cultured them with HLA-C*07:02+CSPG4+ melanoma cell lines (Figure 2E).