Next, to explore whether NT-3 can promote peripheral nerve regeneration by maintaining a high level of c-Jun after chronic denervation, two shRNAs targeting c-Jun (sh1 and sh2) and a control non-interfering shRNA (shCtrl) were constructed and packed into AAV2/9, which has been reported to have high infection efficiency in Schwann cells of rat sciatic nerves [34]. The gene discussed is JUN; the disease is infection.