The S proteins of bat CoVs that are more distantly related to SARS-CoV-2, i.e., Rc-o319, Rm1 and Rf1 detected in R. biasii, R. macrotis and Rf bats, respectively (Fig. 1b), were generally unable to use any ACE2 ortholog for infection (Fig. 3a). This evidence concerns the gene PROS1 and infection.