Tg(wt1b:gfp) embryos injected with SB-MO clic5 or TB-MO clic5b revealed ciliopathy-associated phenotypes such as pronephric cyst formation, defective LR-asymmetry, ventral body curvature, otolith deposition defects and hydrocephalus formation. The gene discussed is CLIC5; the disease is ciliopathy.