In contrast, the CXCR4-specific inhibitors AMD3100 and WZ811 significantly reduced the signs associated with nephrotoxicity in I/R+Met mice, including renal hypertrophy (Fig. 6c, d); increased renal biomarkers such as serum BUN (Fig. 6e), creatinine (Fig. 6f), LDH (Fig. 6g), AST (Fig. 6h) and Lcn2 mRNA levels (Fig. 6i); elevated renal MDA, a marker of ferroptosis‒induced neutrophil mobilization and expansion of neutrophils (Fig. 6j); and renal fibrosis (Fig. 6l). This evidence concerns the gene LCN2 and renal fibrosis.