ACTN4 and melanoma: For example, the Gly795Ala mutation on Eef2 with the core peptide of LPVNESFAF (ranked 11 in Supplementary Table 1) and the Phe835Val mutation on Actn4 with the core peptide of VTFQAFIDV (rank 21 in Supplementary Table 1) for MHC-I were proven to be the functional neoantigens for melanoma.17 For MHC-II, 10 peptides were previously detected as neoantigens in Sebastian’s work,17 and were ranked from rank 28 to rank 122 among 147 MHC-II peptides in Supplementary Table 2.