CRH and breast neoplasm: To address whether the anxiety-induced hyperactivation of the CeMCRH→LPGi circuit alters breast tumor progression, we performed bilateral injection of retrogradely transported retro-AAV expressing Cre recombinase (rAAV2/retro-CRH-Cre) into the LPGi and a Cre-dependent AAV encoding hM4Di-mCherry or mCherry (rAAV2/9-EF1α-DIO-hM4Di-mCherry or rAAV2/9-EF1α-DIO-mCherry) into the CeM (Figure 8, A–C).