Next, to confirm and further examine whether anxiety-induced activation of the CeMCRH→LPGi circuit affects tumor progression, we bilaterally injected a retrograde rAAV2/retro-CRH-Cre virus into the LPGi and a Cre-dependent rAAV2/9-EF1α-DIO-hM3Dq-mCherry or rAAV2/9-EF1α-DIO-mCherry (as a control) virus into the CeM (Figure 9, A–C). The gene discussed is CRH; the disease is Anxiety.