In this study, we injected rAAV2/2-CRH-Cre together with rAAV2/9-EF1α-Flex-taCasp3-TEVp into the CeM to ablate the CRH neurons and used optogenetic and chemogenetic approaches to specifically activate or inhibit CeMCRH neurons to investigate their role in cancer-induced anxiety and tumor progression. The gene discussed is CRH; the disease is cancer.