The u2-os cells are epithelial morphologic cell lines obtained from meso-differentiated sarcomas of the tibia of patients with osteosarcoma; due to the bone metastatic nature of KIRC, we selected the u2-os cell lines for verification, and based on the HPA website, expressions of IKBKB and IKBKG in u2-os cell lines were found. The gene discussed is IKBKB; the disease is sarcoma.